Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal)

Effect of Mutation of RNA-dependent RNA Polymerase (RdRp) of Hepatitis C Virus on Affinities of Dasabuvir: Computational Study

2024-03-05T03:03:22+00:00

Hepatitis C Virus (HCV) is one of the infectious diseases that has posed a serious threat to global public health for the past few decades. HCV is an RNA virus that infects the human liver and can lead to chronic liver damage, cirrhosis, and even liver cancer. Treatment for HCV infection has made rapid advancements in recent years, particularly with the development of more effective antiviral drugs. One of the drugs used in HCV therapy is dasabuvir. Dasabuvir is an RNA-dependent RNA polymerase (RdRp) inhibitor that functions to inhibit the replication of the HCV virus. The RdRp enzyme in HCV is represented by NS5B, and dasabuvir specifically targets this enzyme. Several reports have revealed mutations in HCV NS5B due to the use of dasabuvir. This study conducted a computational mutation analysis on NS5B of HCV resulting from dasabuvir usage. The research findings indicate that mutations in the HCV polymerase induced by dasabuvir usage lead to changes in dasabuvir's conformation and binding energy. Some mutations decrease binding energy, such as mutations C316N, C451S, and N411S. However, on the other hand, there are mutations that increase binding energy, such as M414V, A553V, and C445F. The decrease in binding energy is supported by increased hydrogen bonding interactions with Asp318, Gln446, and Tyr448, as well as the formation of new hydrogen bonds, such as hydrogen bonding with Ser288 in C451S and Arg200 in C451S. Meanwhile, the increase in binding energy is supported by decreased binding interactions with Asp318 and pi-pi interactions with Phe193. Hydrogen bonding with Asn291 also decreases, as seen in A553V, and is even lost in C445F. Future work will be devoted for designing new dasabuvir derivatives which having better affinited to NS5B of HCV.

Effectiveness of Citronella and Basil Essential Oil Spray as Repellents against Aedes aegypti Mosquitoes

2024-03-01T13:27:39+00:00

Background: In 2023, Dengue Fever cases in Indonesia reached 35,694 cases from January to May. Dengue Fever is caused by the bite of Aedes aegypti mosquitoes. Mosquito control efforts have been widely conducted, including the use of chemical insecticides. The use of chemical insecticides can lead to environmental contamination and cause skin irritation. An alternative approach that can be taken is to use natural insecticides. One of the natural insecticides that can be used as a repellent is citronella and basil essential oils. Citronella and basil essential oils contain geraniol and citronellal, which can cause mortality in mosquitoes. This study intends to evaluate the effectiveness of a spray mixture of basil leaves and citronella essential oil as a mosquito repellent. Materials and Methods: Aedes aegypti mosquitoes, basil leaf essential oil, citronella essential oil, propylene glycol, glycerin, butylated hydroxytoluena and etanol 96% v/v. Subsequently, an evaluation of physical properties was conducted, including organoleptic testing, pH, and viscosity evaluation, as well as irritation and repellent activity test. Results: The spray formulations in the control and F1 are clear in color, while F2 and F3 are clear with a slightly yellowish hue. The scent produced has the characteristic smell of citronella. The pH and viscosity tests resulted in pH values consistent with the skin's pH range of 4.5-7 and viscosity within the range of 1-1000 Cp. The spray formulation did not irritate after 48 hours of observation. The results of the effectiveness test indicate that F2 can protect against mosquito bites for 1 hour, while F3 can protect for 3 hours. Conclusion: Citronella essential oil and basil leaves are effective as a repellent for the Aedes aegypti mosquito for 3 hours of use in concentration 15%.

Effects of Selective Serotonin Reuptake Inhibitors Exposure in Stressed Pregnant Mice on the Anxiety Behavior of the Offspring

2024-03-01T13:27:39+00:00

Background: Stress hurts problem-solving abilities, while anxiety disorders and depression are associated with unpleasant feelings and impaired daily functioning. Open field test (OFT) is used to assess anxiety-like behavior in animals by observing their exploratory behavior. Pregnant women are vulnerable to stress, which increases the risk of premature birth and low birth weight. Stress during pregnancy can also lead to preeclampsia and behavioural problems in newborns. Selective serotonin reuptake inhibitors (SSRIs), commonly used antidepressants during pregnancy, help manage mental disorders by increasing serotonin levels and regulating the endocrine system. They reduce glucocorticoid levels, thereby alleviating anxiety and stress in pregnant women. Objectives: This research investigates the correlation between the administration of SSRIs to stress-induced expectant mothers, the manifestation of depression-like behaviour, and the expression of glucocorticoid receptors in the offspring's hippocampus. Material and Methods: This study included four mouse groups: control without stress, control with offspring stress, stress model with footshock on dams and o ffspring, and stress with fluvoxamine treatment. Depressive and stress-related behaviors were measured using OFT. Results: OFT was used to assess the behaviour of offspring mice. Significant differences were observed in the number of crossings in the centre area and behaviour. The SSRI treatment showed potential anxiolytic effects, while stress led to reduced behaviour. These findings contribute to understanding anxiety-related responses and the effects of stress and SSRI treatment in animal models. Conclusions: OFT revealed significant differences in anxiety behaviour, specifically in the number of crossing centres, the centre area, and behaviour. SSRI treatment exhibited anxiolytic effects, while stress-decreased behaviour findings enhanced our understanding of anxiety responses and the impact of stress and SSRI treatment in animal models.

Characteristics of Metformin Transporter Coding Gene (SLC22A1 rs628031 and SLC47A1 rs2289669) in Healthy Indonesian Subject

2024-03-01T13:27:39+00:00

Background: Metformin might be a first therapy for Type 2 diabetes; however, it had a lot of variation in glycemic response. Objective: to analyze the frequency of minor alleles of the OCT1 coding gene (SLC22A1 rs628031) and MATE1 coding gene (SLC47A1 rs2289669) as well as the genotypic variation of the interaction of these two genetic polymorphisms in healthy subjects of Indonesia. Material and Methods: Through inclusion criteria, the study employed an observational descriptive technique with 70 Indonesian Javanese healthy individuals. Subject health information was obtained from the complete blood routine test, serum glutamic oxaloacetic transaminase/ serum glutamic-pyruvic transaminase (SGOT/SGPT), complete urine test, and serum creatinine tests. All data were compared to the normal range. Results: The finding shows that the minor allele frequency on SLC22A1 rs628031 A>G and SLC47A1 rs2289669 G>A respectively at 53% and 36%. There are four SLC22A1 rs628031 and SLC47A1 rs2289669 genetic polymorphism interactions; 16 Wt/Wt (16.75%), 24 Wt/M (25%), 32 M/Wt (33.3%) and 24 M/M (25%). The discovery demonstrates that such minor allele frequencies of the SLC22A1 rs628031 OCT1 and SLC47A1 rs2289669 MATE1 genes in healthy Indonesian subjects are relatively high. The SLC22A1 rs628031 A>G and SLC47A1 rs2289669 G>A respectively at 53% and 36%, almost the same as the minor allele frequencies found in several other Asian countries eg. India, Japan, and China. Conclusions: OCT1 coding genes (SLC22A1 rs628031 A>G) were found more dominant than MATE1 coding genes (SLC47A1 rs2289669 G>A) and both alleles are relatively high in healthy subjects of Indonesia, which can be used as information to explore the consequences of different genes' interactions on the Indonesian pharmacokinetic properties, and the efficacy variations of metformin in Type2 DM patients.

Lipase Enzyme Inhibitory Activity of Jombang Leaves Extract (Taraxacum officinale F.H. Wigg)

2024-03-01T18:12:59+00:00

Background: Obesity is a condition that occurs due to an imbalance between energy intake and expenditure. Lipase enzymes play an important role in the process of fat metabolism, making it a target in obesity treatment strategies. Jombang plant has been used for generations as an alternative treatment for various diseases. The main content of secondary metabolite compounds in jombang plants are phenolics, flavonoids, terpenoids, and alkaloids. Objective: This study was conducted to determine the activity and potential inhibition of jombang plant (Taraxacum officinale F.H. Wigg.) against lipase enzyme in vitro. Materials and Methods: The phytochemical screening was determined by a color change reaction with certain reagents, while total phenolic content was carried out using Folin-Ciocalteau reagent spectrophotometrically. The inhibitory activity of 96% ethanol extract of jombang leaves as well as orlistat as positive control was measured by titration method and using olive oil substrate. The extract concentrations used were 125 ppm, 250 ppm, and 500 ppm. Results: Jombang leaf extract contains alkaloid, phenolic, flavonoid and terpenoid compounds qualitatively. Determination of total phenolic content in 96% ethanol extract of jombang leaves was obtained at 12.69 ± 0.91 mgGAE/g. The highest percentage of inhibition was found at a concentration of 500 ppm which amounted to 103.85%. The difference in the percentage of inhibition in all groups showed no significant difference (Sig>0.05). Conclusion: This study shows that jombang leaf extract is able to inhibit lipase enzyme activity with the highest percentage of inhibition was 103,85%.

Analysis of the Effectiveness of Drug Management Systems in Tangerang Selatan General Hospital in 2021

2024-03-02T03:48:33+00:00

Background: Pharmaceutical services are closely related to an optimal drug management system, where with optimal drug management, drug availability can be guaranteed, and patient demand can be fulfilled. Objectives: This study aimed to analized the effectiveness of the drug management system at the distribution stage at the Tangerang Selatan General Hospital in 2021. Material and Methods: The method used wasbased on a book by Satibi in the form of percentage of compatibility of drugs with drug stock cards, Turn Over Ratio (TOR), drug availability levels, percentage of expired and damaged drugs, and percentage of dead stock as measured usedstandards. Data taken prospectively for compatibility of drugs with drug stock cards, and retrospectively for other indicators by looking at generic drug stock cards in 2021. Results: The results showed that the drug compatibility indicators with drug stock cards and the level of availability of drugs were in accordance with the standards, while the Turn Over Ratio (TOR) and dead stock indicators did not meet the standard indicators. Conclusions: The drug management system at the South Tangerang City General Hospital can still be improved.

Characteristics of Hyloceregurt: Fermented Milk from Stems and Peel of Dragon Fruits

2024-03-02T18:13:29+00:00

Background: Fruitghurt, the result of fruit or fruit by-product fermentation, is a functional food. Functional foods are foods that are rich in benefits. A formulation of hyloceregurt has been derived from the stem and peel of the dragon fruit (Hylocereus polyrhizus), with potential as an immunomodulatory agent. Objectives: This study is aimed at determining the quality of hyloceregurt. Material and Methods: Hyloceregurt formula variations are made from fermented stem powder, fruit peel powder, or dragon fruit peel juice. The test include physical characteristics, chemical analysis, microbiology analysis, and hedonic test. Results: Hyloceregurt formula from the stems of the plant and the peel of the dragon fruit has almost the same physical characteristics that include a distinctive smell, an acidic taste with a pH ranging from 3-5, and a thick liquid consistency. The color of the hyloceregurt follows the waste of the dragon fruit used (pink and green). The chemical characteristics of hyloceregurt have met the SNI standard, including fat content with a range of 3.1-3.6% and protein content with a range of 4.95–6.74% b/b. The microbiological characteristics of hyloceregurt indicate that the value of the total plate figure is in the range of 1.1-3.3 x 10⁹ CFU/mL. Panelists on the hedonic test preferred three formulas: 1% hyloceregurt from the peel powder or fresh peel fruit juice, and dragon fruit plant stems based on smell, taste, color, and consistency. Conclusions: All formulas in 1% fruit or by-fruit were the most preferred formulas, and based on the best physical characteristic tests and chemical content analysis, met the requirements of SNI 2981 of 2009. However, microbiologically, they still did not meet the requirements of SNI 2981 of 2009, so further tests still need to be carried out.

Enhanced Fibrogenesis Activity in Diabetic Wound Using 20% Anredera scandens (L.) Moq. Leaves Extract Ointment in Sprague dawley Rats

2024-03-02T18:13:14+00:00

Background: In diabetic conditions, decreased glucose-6-phosphate dehydrogenase activity results in reduced nitric oxide synthesis. Reduced nitric oxide synthesis results in the production of ROS. This may impede the processes of angiogenesis and vasoconstriction and prolong inflammation. Objective: We were interested in investigating the mechanism by which 20% of Anredera scandens leaves extract ointment increases fibrogenesis activity because this condition can affect the development of damaged skin tissue. This study's design is a post-test-only laboratory experiment. Material and methods: The study employed male Sprague dawley strain rats that were induced with 40 mg/kgBW of STZ. The mice were divided into four groups: the negative group (which got diabetic wounds only), the positive group (which got Gentamicin ointment), and the test group (which got 20% extract ointment). Results: The skin tissue from each group was then examined histopathologically. After receiving 20% ointment of Anredera scandens leaves extract, the test group's mean fibrogenesis histopathology score on day 14 was significantly higher (P=0.022) at 3.75 than that of the negative group, which had a mean score of 2.75. Following that, the skin tissue of both the extract test group and the normal group was examined histopathologically. In comparison to the normal group mean score of 4.0 on day 14, the test group, which received 20% ointment of Anredera scandens leaves extract, had a non-significant (P=0.317) mean fibrogenesis histopathology score of 3.75. Subsequently, skin tissue was examined histopathologically for both the positive and normal groups. According to the results, on day 14, the positive group's average fibrogenesis histopathology score was 3.5, while the normal group's average score was 4.0. This difference was not statistically significant (P=0.127). Conclusions: Thus, it can be said that 20% Anredera scandens leaf extract ointment is comparable to gentamicin ointment in its capacity to promote fibrogenesis activity in diabetic wounds.

Evaluation of Anti-diabetic Drugs using ATC/DDD and DU90% Methods in Diabetes Mellitus Patients

2024-03-04T18:14:33+00:00

Background: Diabetes Mellitus (DM), which results from insulin resistance, is closely linked to long-term damage to pancreatic beta cells, organ dysfunction, and organ failure, particularly in the eyes, kidneys, nerves, heart, and blood vessels. The development of the ATC/DDD system is necessary to increase global drug knowledge, ensure equitable drug availability, and promote responsible drug use. Objectives: This study aimed to identify data on patient characteristics and oral anti-diabetic drugs and insulin using the ATC/DDD and DU90% methods. Material and Methods: This study was a cross-sectional evaluation of oral anti-diabetic medications and insulin using the ATC/DDD method and DU90% at the Bala Keselamatan Bokor Turen Hospital from January to December 2022. The inclusion criteria were patients diagnosed with type 1 or type 2 diabetes mellitus, with or without comorbidities, who were treated with oral anti-diabetic medications and a combination of insulin and oral medications. In the present study, the incomplete medical record data served as the exclusion criteria. The sample for this investigation consisted of 238 patients selected using the complete sampling technique. Results: Most patients who used oral anti-diabetic medications and insulin were between the ages of 46 and 65 (71.34%), were female (73.2%), had standard body mass index (BMIs between 18.5 and 25), and had diagnoses of DM + HT (20.73%) and Type II DM (18.29%). Glulisin was the most used anti-diabetic drug, accounting for 589 DDD/100 days of hospitalization, and DU90% was 21.39%. Conclusion: Glulisin is the most frequently prescribed anti-diabetic medication at Bokor Turen Hospital.

Effects of Pamelo Orange Peel (Citrus maxima) Ethanol Extract on Macroscopic Kidney and Heart of Hypertensive Rats

2024-03-04T18:14:20+00:00

Background: Hypertension, characterized by elevated blood pressure (≥140/90 mmHg), leads to kidney and heart damage. Hypertension causes increased heart rate, heart enlargement, risk of heart failure, and damage to kidney blood vessels. One way to treat hypertension is by administering drugs such as herbal medicines. Pamelo oranges (Citrus maxima) are starting to be developed in the treatment of diseases. The ethanol extract of pomelo orange peel (EEKJP) contains flavonoid compounds which have antihypertensive activity as a natural Angiotensin Converting Enzyme (ACE) inhibitor, diuretic, increases Nitric Oxide Synthase (NOS) activity and activates Endothelium Derived Relaxing Factor (EDRF). Objectives: This study aims to examine the effect of ethanol extract of pomelo orange peel on the macroscopic features of the heart and kidneys of hypertensive rats (Rattus norvegicus). Methods: This research uses The Posttest-Only Control Group Design. Thirty rats were divided into six groups, namely naive control, negative control (NaCMC 1%), positive control (Captopril 0,45 mg/200 gramBB), EEKJP 100 mg/KgBW group, EEKJP 150 mg/KgBW group, and EEKJP 200 mg/KgBW group. Hypertension condition through induction with prednisone 1.5 mg/KgBW and NaCl 2% for 21^th days. The test preparation was administered orally for 14^th days. Next, all rats were dissected to remove the heart and kidney organs for macroscopic observations. Results: The results of the study showed that ethanol extract of pomelo (Citrus maxima) orange peel had a varied effect on the macroscopic appearance of the heart and kidneys of rats with hypertension. The heart organ shows structural improvements, while the kidneys have not shown any improvement. Conclusions: Extract with a dose of 100 mg/KgBW has no changes in the macroscopic appearance of the heart so it is thought to be able to repair damage to the heart organ.

Molecular Docking and Molecular Dynamic Studies of Secondary Metabolites from Momordica Charantia as Natural Antidiabetic

2024-03-11T18:20:50+00:00

Background: Diabetes Mellitus is a non-contagious disease characterized by hyperglycemia. Diabetes mellitus occurs when the body cannot receive or use insulin properly. If you already have diabetes, then the patient must take medication continuously because diabetes mellitus is a lifelong disease. Because medicines are quite expensive, alternative ways to cure the disease are needed by consuming traditional medicines, one of which is bitter melon (Momordica charantia). Objectives: This research aims to predict the secondary metabolite compounds in the bitter melon plant, analyze molecular interactions, and identify compounds that can lower blood sugar levels. Material and Methods: 12 compounds from the Momordica charantia plant and six proteins (1IR3, 1RHF, 1XU7, 4PNZ, 4YVP, 2NT7) that will be docked using Pyrx and Yasara Dynamics applications. Results: From the molecular docking results, three compounds with the highest binding affinity were found in Momordica charantia: momordenol, oleanolic acid, and momordicin. Based on molecular dynamics simulations, these three compounds were stable in their interactions with the six proteins tested, namely 1IR3, 1RHF, 4PNZ, 4YVP, 1XU7, and 2NT7. Momordenol and momordicin showed the most stable interaction profiles. Furthermore, ADMET tests showed that momordenol, oleanolic acid, and momordisin have drug-like characteristics. Conclusions: The Momordica charantia plant has the potential to act as an antidiabetic agent.

Analysis of Therapy Satisfaction on the Quality-of-Life of Type 2 Diabetes Mellitus Patients at Anutapura Hospital Palu

2024-03-31T18:39:08+00:00

Background: Type 2 Diabetes Mellitus (Type 2 DM) is a chronic disease characterized by increased blood glucose levels caused by impaired insulin function. In Palu City, Type 2 DM is the 2nd most common in Central Sulawesi with a total of 26,204 people. Objective: This study aims to determine the relationship between therapy satisfaction and the quality of life of Type 2 DM patients who perform outpatient treatment at Anutapura General Hospital, Palu. Methods: With a random sample approach, 100 respondents were used in this cross-sectional study that employed an observational method. Data collection used instruments in the form of a DMSAT (Diabetes Medication Satisfaction Tool) therapy satisfaction questionnaire and a DQOL (Diabetes Quality Of Life) quality of life questionnaire. Results: The results of the study were obtained on therapy satisfaction from Type 2 DM patients with the number of respondents in the very satisfied (1.0%), satisfied (90.0%), and quite satisfied (9.0%) categories while the results of the study of the quality of life of Type 2 DM patients 2 fall into the good category (38.0%), and the bad category (62.0%). The statistical analysis test used was the Spearman Rank Correlation test. The results of the Spearman Rank Correlation test value p = 0.037 or p <α (0.05), then H0 is rejected, which means that there is a relationship between satisfaction with therapy and quality of life in patients with Type 2 DM which is very weak. Conclusion: The conclusion in this study is that there is a very weak relationship between therapy satisfaction and quality of life of patients.