Main Article Content


Background: The condition of hyperglycemia in Diabetes mellitus (DM) causes a disruption of the function of phagocytes that can facilitate M. tuberculosis infection. Immunostimulant from natural ingredients can help improve the condition of hyperglycemia an immune function.

Objective: To analyze the effect of Hibiscus rosa-sinensis L. extract on fasting blood glucose level and the number of mast cell in TB-DM mouse model.

Methods: This experimental study used 27 male Wistar rats, 8 weeks of age, 200-230 g weight, divided into 3 groups, K: Normal control group, P1: TB-positive mouse group P2: Group rat TB-DM + Hibiscus rosa-sinensis L extract dose 250 mg/kgBW and 3 time measurement of H + 7 DM, H + 14 DM and H + 21 DM. TB-DM model mice were prepared with the administration of nicotinamide (NA) 100 mg/kgBW and streptozotocin (STZ) 65 mg/Kg BW, TB agent 1.5 x 105 CFU. Fasting blood glucose levels were measured by GOD-PAP method and Pulmonary tissue is excised, made in paraffin blocks and stained with blue Toluidine to count the mast cells.

Results: There was an improvement in the mean fasting blood glucose levels in the group treated with the Hibiscus rosa-sinensis L. extract of 250 mg/kgBW compared to the TB-DM group without extract therapy followed by decreased the number of mast cell.

Conclusion: Hibiscus rosa-sinensis L. extract. improves fasting blood glucose levels and decreases the number of mast cell of TB-DM rat model.


Hibiscus rosa-sinensis L. TB-DM blood glucose level mast cell

Article Details

Author Biographies

David Pakaya, Universitas Tadulako

Departemen Histologi Fakultas Kedokteran Universitas Tadulako, Palu

Marzela Dewi, Sekolah Tinggi Ilmu Farmasi Pelita Mas

Program Studi Ilmu Farmasi, Sekolah Tinggi Ilmu Farmasi Pelita Mas, Palu

Arlita Leniseptaria Antari, Universitas Diponegoro

Departemen Mikrobiologi Fakultas Kedokteran Universitas Diponegoro, Semarang

Indah Saraswati, Universitas Diponegoro

Departemen Kimia Fakultas Kedokteran Universitas Diponegoro, Semarang
How to Cite
Pakaya, D., Dewi, M., Antari, A. L., & Saraswati, I. (2020). Ekstrak Hibiscus rosa-sinensis L. Memperbaiki Kadar Glukosa Darah Puasa dan Jumlah Sel Mast pada Model Tikus Diabetes Terinfeksi Mycobacterium tuberculosis: Hibiscus rosa-sinensis L. Extract Improves Fasting Blood Glucose Levels and Number of Mast Cells in Diabetic Rat Model that Infected with Mycobacterium tuberculosis. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 6(1), 14 - 19.