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Feb 4, 2020
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Mar 14, 2020
Published
Mar 17, 2020
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Abstract

Songi (Dillenia serrata) is a tree endemic to Southeast Sulawesi and its stem bark been used in folk medicine. Nonetheless, only a handful scientific knowledge regarding chemistry and biological activities has been investigated on the plant. The present study aimed to investigate the antioxidant and xanthine oxidase (XO) inhibitory activity of the stem bark of the plant. Methanol extract and organic fractions (petroleum ether, ethyl acetate, and methanol) of the dried powdered stem bark of songi were evaluated for phytochemical screening, total phenolic (TPC), total flavonoid contents (TFC), DPPH, FRAP, and XO in vitro assays. Flavonoids, tannins, terpenoids, steroids, and saponins present in the extract. TPC and TFC in extract were 59.2 mg GAE/g and 23.4 mg QE/g. The contents in organic fractions were in solvent-dependent manner (methanol>ethyl acetate>petroleum ether). Extract and fractions scavenged DPPH radicals (48.2–59.7%) at 100 μg/mL compared to ascorbic acid, trolox, and gallic acid (90.3–93.8%). FRAP values varied from 0.8–3.4 μg/μg equivalent trolox amount (quercetin and gallic acid were 25.7 dan 32.4 μg/μg, respectively). They also inhibited xanthine oxidase (15.3–50.3%) at 100 μg/mL (allopurinol, 98.2%). The study concluded the potential of methanol extract and organic fraction of the stem bark of songi, thus highlighted the prospect of songi to be used in herbal and drugs development from nature.

Keywords

Dilleniaceae Dillenia serrata Thunb. Fenolik Flavonoid Antioksidan Xantin Oksidase

Article Details

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Author Biographies

Juriyati Jalil, Universiti Kebangsaan Malaysia

Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.

Norizan Ahmat, Universiti Teknologi MARA

Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam, Selangor, Malaysia.

Nor-Ashila Aladdin, Universiti Kebangsaan Malaysia

Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.

Harni Sartika Kamaruddin, Universitas Sembilanbelas November Kolaka

Program Studi Farmasi, Fakultas Sains dan Teknologi, Universitas Sembilanbelas November Kolaka, Kolaka, Indonesia.

Retno Wahyuningrum, Universitas Sembilanbelas November Kolaka

Program Studi Farmasi, Fakultas Sains dan Teknologi, Universitas Sembilanbelas November Kolaka, Kolaka, Indonesia.

How to Cite
Sabandar, C. W., Jalil, J., Ahmat, N., Aladdin, N.-A., Kamaruddin, H. S., & Wahyuningrum, R. (2020). Aktivitas Antioksidan dan Penghambatan Xantin Oksidase Kulit Batang Songi (Dillenia serrata Thunb.): Antioxidant and Xanthine Oxidase Inhibitory Activity of Stem Bark of Songi (Dillenia serrata Thunb.). Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 6(1), 151 - 159. https://doi.org/10.22487/j24428744.2020.v6.i1.15008

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