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The present study aims to optimize the natural deep eutectic solvent (NADES) as a green solvent-based microwave-assisted extraction (MAE) of total polyphenols content (TPC) from Mitragyna speciosa Korth Havil leaves using response surface methodology (RSM). Preparation of natural deep eutectic solvent (NADES) as a green solvent was performed by melting the two-component of malic acid and glucose using a magnetic stirrer. The leaves sample was extracted using the NADES-MAE method with various extraction conditions with four factors and three levels (Box Behnken Design) and optimized using RSM with licensed software of Design Expert V12. TPC was determined using a Folin-Ciocalteau reagent and absorbance was measured on a UV-VIS spectrophotometer at 770 nm and gallic acid as a standard. This study was obtained the optimum extraction conditions include: NADES ratio (malic acid: glucose) of 1:2 g/g, 50% microwave power, a solvent-sampel ratio of 12:1 mL/g, and extraction time for 7 minutes. The optimum conditions were obtained according to the equation formula: TPC = 165.17 – 33.97X1 + 38.36X2 – 6.08X3 + 16.12X4 – 62.77X1X2 + 79.61X12X2, where a R2 value = 0.7102 with a TPC prediction of 402.087±43.81 mg GAE/g sample. These conditions can be used to extract TPC from this plant efficiently, quickly, easily, and environmentally friendly.


Box Behnken Design Mitragyna speciosa Kort Havil Natural Deep Eutectic Solvent Response Surface Methodology Total Polyphenols Content

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How to Cite
Ahmad, I., Yusniah, A., Nur, Y., Prabowo, W. C., & Herman. (2020). Pengayaan Polifenol Total dari Daun Kadamba Menggunakan Metode Ekstraksi Berbantu Mikrowave Berbasis Pelarut Hijau: Total Polyphenols Enrichment from Mitragyna speciosa Korth Havil Leaves Using Green Solvent Based Microwave-assisted Extraction Method. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 6(2).


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