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Submitted
Mar 19, 2020
Accepted
Aug 14, 2020
Published
Sep 30, 2020
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Abstract

Heat shock proteins-90 (HSP-90) is a protein that plays an important role in the life cycle of normal and cancer cells for their self protection from thermal stress, oxidative damage, and cell hypoxia. Inhibition of HSP90 is one way to suppress the growth of cancer cells. In this study, pharmacophore modeling and molecular docking were conducted to identify hit compounds as inhibitors of HSP-90. The pharmacophore feature consists of three hydrogen bond acceptors, one hydrogen bond donor and one hydrophobic feature with Area Under Curve of Receiver Operating Characteristics (AUCROC) is 0.5 and the Goodness of Hit (GH) value is 0.752. Screening in the ZINC database generated 1,500 hit compounds, were subjected to molecular docking to determine their binding energy and interactions with HSP-90. The range of binding energy (E) of all hit compounds is -5.68 to -12.24 kcal/mol and there are four best hit compounds namely lig_543, lig_527, lig_1337 and lig_337, when compared to native ligands (PU2, E=-8.25 kkal/mol) based on the binding energy and orientation, which indicate their potential as new HSP-90 inhibitors.   


 

Keywords

Pharmacophore Heat Shock Protein-90 Molecular Docking Virtual Screening

Article Details

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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Author Biographies

Muhammad Arba, Universitas Halu Oleo

Fakultas Farmasi, Universitas Halu Oleo, Kendari, Indonesia.

Arfan, Universitas Halu Oleo

Fakultas Farmasi, Universitas Halu Oleo, Kendari.

Ayu Trisnawati, Universitas Halu Oleo

Jurusan Kimia, Universitas Halu Oleo, Kendari.

Desi Kurniawati, Universitas Halu Oleo

Jurusan Kimia, Universitas Halu Oleo, Kendari.

How to Cite
Arba, M., Arfan, Trisnawati, A., & Kurniawati, D. (2020). Pemodelan Farmakofor untuk Identifikasi Inhibitor Heat Shock Proteins-90 (HSP-90): Pharmacophore Modeling to Identify Heat Shock Proteins-9 (HSP-90) Inhibitors. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 6(2). https://doi.org/10.22487/j24428744.2020.v6.i2.15036

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