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Aug 2, 2020
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Sep 28, 2020
Published
Sep 30, 2020
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Abstract

Xestospongia sp. is one of marine sponge belongs to demosponges class that mainly found in Southeast Sulawesi and the secondary metabolites contained in Xestospongia sp. suspected to have immunomodulatory activity. A previous study exhibited the immunomodulatory of Xestospongia sp. ethanolic extract (XEE) at dose of 300 and 400 mg/Kg BW by affecting the phagocytic activity of macrophages. Thus, this study aims to investigate the effect of XEE towards interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) at dose of 300 and 400 mg/Kg BW. Wistar male rats are divided into 4 groups (n=6) randomly and treated for 7 days orally each as follow: group I (XEE dose of 300 mg/KgBW); group II (XEE dose of 400 mg/KgBW); group III (0.5% NaCMC); and group IV (commercial phylantii extract). On day 8, animals were infected with Staphylococcus aureus and left for 1 hour. Animals were sacrificed and the blood was drawn by cardiac puncture (3 mL), followed by analyzed under ELISA Kit for IFN-γ and TNF-α. Collected data were analyzed statistically using SPSS®. The IFN-γ levels obtained were 350.113; 392.970; 118.416; and 61.958 ρg/mL, respectively and the TNF-α were 2808; 1308; 778; and 845.5 ρg/mL, respectively. According to results obtained, both doses of XEE are affecting the IFN-γ and TNF-α levels (p<0.05) compared to group III as negative control, and group IV as positive control. As conclusion, XEE of both doses is increasing IFN-γ and TNF-α levels of animals that respond to phagocytic activity

Keywords

Xestospongia sp Immunomodulatory IFN-γ TNF-α.

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Author Biographies

Jumadil, University of Halu Oleo

Faculty of Pharmacy, University of Halu Oleo, Kendari.

Wahyuni, University of Halu Oleo

Faculty of Pharmacy, University of Halu Oleo, Kendari.

Muh. Hajrul Malaka, University of Halu Oleo

1Faculty of Pharmacy, University of Halu Oleo, Kendari.

Wa Ode Harnita, University of Halu Oleo

Faculty of Pharmacy, University of Halu Oleo, Kendari.

Baru Sadarun, University of Halu Oleo

Faculty of Fisheries and Marine Science, University of Halu Oleo, Kendari.

Agung Wibawa Mahatva Yodha, University of Halu Oleo

Faculty of Pharmacy, University of Halu Oleo, Kendari.

Saripuddin, University of Halu Oleo

Faculty of Pharmacy, University of Halu Oleo, Kendari.

La Ode Muhammad Julian Purnama, University of Halu Oleo

Faculty of Pharmacy, University of Halu Oleo, Kendari.

How to Cite
Fristiohady, A., Jumadil, Wahyuni, Malaka, M. H., Harnita, W. O., Sadarun, B., Yodha, A. W. M., Saripuddin, Purnama, L. O. M. J., & Sahidin, I. (2020). Immunomodulatory Activity of Xestospongia sp. Ethanolic Extract Towards Interferon-gamma (IFN- γ) and Tumor Necrosis Factor-alpha (TNF-α) Levels in Wistar Male Rats. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 6(2). https://doi.org/10.22487/j24428744.2020.v6.i2.15231

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