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Aug 30, 2020
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Jan 11, 2021
Published
Mar 1, 2021
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Abstract

Stem cells are potentially used as a regenerative therapy agent. Cell encapsulation is one of the developed methods to utilize Mesenchymal Stem Cells (MSCs) for therapy. This research aimed to study the effect of encapsulation using alginate-CaCl2 towards the viability of hAdMSCs during in vitro culture. Encapsulation of hAdMSCs with alginate-CaCl2 was done using the extrusion method. The viability of hAdMSCs was analyzed with Live/Dead Assay and MTT assay. The results indicated that cultured hAdMSCs within alginate remain alive for 7 days culture period. However, the viability was lower than the reference culture. The absorbances from MTT assay of encapsulated MSCs were lower than the conventional hAdMSCs culture. This result indicated lower metabolic activity of hAdMSCs when cultured in alginate beads.

Keywords

mesenchymal stem cells encapsulation alginate viability

Article Details

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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Author Biographies

Marsudi Siburian, Institut Teknologi Sumatera

Biomedical Engineering Study Program

Sismindari, Universitas Gadjah Mada

Professor in Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Universitas Gadjah Mada

Rilianawati, Badan Pengkajian dan Penerapan Teknologi (Agency for the Assessment and Application of Technology)

Center for Pharmaceutical and Medical Technology

How to Cite
Siburian, M., Sismindari, & Rilianawati. (2021). The Encapsulation Effect on Viability of Mesenchymal Stem Cells: Pengaruh Enkapsulasi Terhadap Viabilitas Sel Punca Mesenkimal. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 7(1), 1-9. https://doi.org/10.22487/j24428744.2021.v7.i1.15258

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