Article Sidebar

Submitted
Oct 30, 2021
Accepted
Mar 3, 2022
Published
Mar 24, 2022
Download
PDF
Statistic
Read Counter : 757 Download : 718

Main Article Content

Abstract

Background: Diabetes mellitus is a chronic disease caused by the body's incapacity to use insulin or the inability of the pancreas to produce insulin. Pandan tikar, belong to Pandanaceae plants, has been known for its benefits. Chemical compounds in pandan tikar leaves showed that it has a lot of medicinal activities, one of them is to lower glucose blood level in diabetes mellitus. Objectives: This study aimed to determine the activity of ethanolic extract of pandan tikar (Pandanus tectorius) leaves as antidiabetic agent in white male rats induced by alloxan. Material and Methods: This study used a pretest-posttest design, with 25 Wistar white male rats. The rats were divided into five groups, each consisting of 5 rats. Group I, a positive control, was given glibenclamide with a dosage of 0.45 mg/kg BW. For the negative control, Group II, Na-CMC 1% was used. The test groups were Group III, IV, and V, and each received an ethanolic extract of pandan tikar leaves with various dosages of 125, 250, and 375 mg/kg body weight (BW), respectively. The rats were conditioned in diabetic stage by using alloxan 150 mg/kg bw as an inducer, given intraperitoneally, then each rats treated accordingly to their own group for 14 days. The determination of blood glucose level was done enzymatically by using glucometer. Result: The result showed that ethanolic extract of pandan tikar leaves able to lower the glucose blood level and not significantly different from glibenclamide. The most optimal dose of pandan tikar leaves ethanolic extract was 375 mg/kg bw, with the decreasing percentage was 56.72%. Conclusions: The ethanolic extract of pandan tikar leaves has antihyperglicemic ability on alloxan-induced diabetic white male rats.

Keywords

Pandanus tectorius Diabetes mellitus Hyperglycemia Alloxan

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Author Biographies

Magi Melia Tanggu Rame, Universitas Citra Bangsa

Jurusan Farmasi, Fakultas Kesehatan, Universitas Citra Bangsa, Kupang Indonesia.

Yohana K. A. Mbulang, Universitas Citra Bangsa

Jurusan Farmasi, Fakultas Kesehatan, Universitas Citra Bangsa, Kupang Indonesia.

How to Cite
Tanggu Rame, M. M., & Mbulang, Y. K. A. (2022). Antidiabetic Activity of Ethanolic Extract of Pandan Tikar (Pandanus tectorius) on Alloxan-Induced Diabetic White Male Rats: (Uji Aktivitas Antidiabetes Ekstrak Etanol Daun Pandan Tikar (Pandanus tectorius) pada Tikus Putih Jantan Galur Wistar yang Diinduksi Aloksan). Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 8(1), 41-51. https://doi.org/10.22487/j24428744.2022.v8.i1.15674

References

  1. Aba, P.E., Isaac U.A., 2018, ‘Mechanisms of actions of some bioactive anti-diabetic principles from phytochemicals of medicinal plants: A review’, Indian Journal of Natural Products and Resources. 9, 85-96.
  2. Atihuta., Frangki., 2018, ‘Uji Aktivitas Ekstrak Kombinasi Batang Dan Daun Suruhan (Piperumia Pellucida L.H.B Kunth) Sebagai Anti Diabetes Pada Tikus Putih’, Jurnal Mitra Pendidikan (JMP Online)’, 2, 205-216.
  3. Azwanida NN., 2015, ‘A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation. Medicinal & Aromatic Plants’, 4, 2167-0412.
  4. Fajriaty, I., Hariyanto., Andres., Setyaningrum, R. 2018. Skrining Fitokimia dan Analisis Kromatografi Lapis Tipis dari Ekstrak Etanol Daun Bintangur (Callophyllum soulattri Burm. F). Jurnal Pendidikan Informatika dan Sains. 7, 54-67.
  5. Ighodaro, O.M., Abiola M.A., Oluseyi, A.A. 2017, ‘Alloxan-induced diabetes, a common model for evaluating the glycemic-control potential of therapeutic compounds and plants extracts in experimental studies’, Medicina. 53, 365-374. https://doi.org/10.1016/j.medici.2018.02.001
  6. Indrayani, S., Resmi M. 2020, ‘Review Artikel : Aktivitas Antidiabetes Beberapa Tanaman Di Indonesia’, Farmaka. 18, 58-65.
  7. Kido Rahelin, et al., 2019, ‘Uji Aktivitas Analgesik Ekstrak Etanol Daun Pandan Tikar (Pandanus tectorius) Pada Mencit (Mus musculus) yang Diinduksi Asam Asetat. Fakultas Farmasi’, Skripsi, Universitas Citra Bangsa.
  8. Kumar, S., Seema, D., Pushpander, K., et al., 2017, ‘Antifertility Effect of Hydroalcoholic Extract of Pandanus Odoratissimus L. Leaves’. Porto Biomedical Journal. 2,167-169. doi: 10.1016/j.pbj.2017.03.001
  9. Lenzen, S., 2008. ‘The mechanisms of alloxan- and streptozotocin-induced diabetes’, Diabetologia. 51, 216-226. DOI 10.1007/s00125-007-0886-7
  10. Ojiako, Okey A., Paul C.Chikezie., Agomuo C.Ogbuji., 2016, ‘Blood glucose level and lipid profile of alloxan-induced hyperglycemic rats treated with single and combinatorial herbal formulations’, Journal of Traditional and Complementary Medicine. 6, 184-192.
  11. Rahmi, H., 2014, ‘Aktivitas Ekstrak Daun Wungu (Graptophyllum pictum (L.) Griff) Dalam Menurunkan Kadar Glukosa Darah Tikus Hiperglikemia’, Thesis, Pasca Sarjana Institute Pertanian Bogor
  12. Riskesdas., 2018, ‘Laporan Nasional Riskesdas 2018’, Kementerian Kesehatan Republik Indonesia, Badan Penelitian dan Pengembangan Kesehatan.
  13. Saifudin, A., 2014, Senyawa Alam Metabolit Sekunder Teori, Konsep, dan Teknik Pemurnian. Edisi 1. Cetakan 1. Deepublish: Yogyakarta.
  14. Sanjeeva., Raveendra, K.N, Padmalaxmi, D., et al., 2011, ‘Antioxidant Activity Of Methanol Extract Of Pandanus Fascicularis Lam’, Pharmacologyonline. 1, 833-841.
  15. Sandhar, H.K., Bimlesh, K., et al., 2011, ‘A Review of Phytochemistry and Pharmacology of Flavonoids’, Internationale Pharmaceutica Sciencia. 1, 25-41.
  16. Sarian, M.N., Qamar U.A., et al., 2017, ‘Research Article: Antioxidant and Antidiabetic Effects of Flavonoids: A Structure-Activity Relationship Based Study’, BioMed Research International.
  17. Soegondo, S., Soewondo, P, Subekti, I., 2009, Penatalaksanaan Diabetes Melitus Terpadu, Jakarta: Fakultas Kedokteran Universitas Indonesia.
  18. Sopianti, D.S., Sary, D.W., 2018, ‘Skrining Fitokimia dan Profil KLT Metabolit Sekunder dari Daun Ruku-ruku (Ocimum tenulforum L.) dan Daun Kemangi (Ocimum sanctum L.)’, Jurnal Farmasi dan Kesehatan. 8, 44-52.
  19. Suarsana, I.N., Iwan H. Utama., I Gusti Agung., Ayu Suartini., 2011, ‘Pengaruh Hiperglikemia dan Vitamin E pada Kadar Malonaldehida dan Enzim Antioksidan Intrasel Jaringan Pankreas Tikus’, Fakultas Kedokteran Hewan, Universitas Udayana.MKB 3
  20. Utomo, A.D., Wiranti S.R., Binar, A.D., 2009, ‘Pengaruh Beberapa Metode Pengeringan Terhadap Kadar Flavonoid Total Herba Sambiloto (Andrographis Paniculata)’, Fakultas Farmasi Universitas Muhammadiyah Purwokerto. 6, 1693-3591.
  21. WHO. 2016. Global Report On Diabetes. France: World Health Organization.
  22. Wulandari D., 2004, ‘Identifikasi Secara Farmakognostik Daun Pandan Duri (Pandanus tectorius Sol.)’, Skripsi. Fakultas Farmasi, Universitas Sanata Dharma.
  23. Yadav, R.N.S., Agarwala, M., 2011, ‘Phytochemical Analysis of Some Medicinal Plants’, Journal of Phytology. 3, 10-14.