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Abstract
Background: Diabetic gangrene is a complication of Diabetes mellitus caused by Staphylococcus aureus. The combination of Ceftriaxone and Chloramphenicol is often used to cure gangrene infection, even though, they produce antagonist interaction based on theory. Objectives: To evaluate the potency of Ceftriaxone, Chloramphenicol and its combination on Staphylococcus aureus isolate of Diabetic gangrene. Material and Methods: The research was done by using disc diffusion methods with Muller Hinton media. Ceftriaxone, Chloramphenicol and its combination dose of 7,5 µg/ml, 15 µg/ml and 30 µg/ml, respectively were tested on Staphylococcus aureus culture taken form the diabetic gangrene patients. Antibacterial effect was observed by measuring inhibition zone on bacteria culture. Type of interaction was analyzed by Ameri-Ziaei Double Antibiotic Synergism Test (AZDAST) method. The results of study were tested statistically with One Way ANOVA (p=0.05) followed by Least Significant Difference (LSD) test. Results: The combination of Ceftriaxone and Chloramphenicol showed an antibacterial effect lower than Ceftriaxone. ß-lactam antibiotic like Ceftriaxone require the cell be growing and dividing in order to have a bactericidal action. Meanwhile, Chloramphenicol causes a slow growth of Staphylococcus aureus and impairs bactericidal effect of Ceftriaxone if they are combined. Conclusions: Ceftriaxone and Chloramphenicol combination has lower antibacterial effect than the single antibiotic groups on Staphylococcus aureus isolate of Gangrene diabetic and the type of interaction is antagonistic.
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References
- Adam, J. M. F. (1985). Foot Diabetic. Cermin Dunia Kedokteran. No 39.
- Brand, P. W. (2000) The foot in diabetes in Rifkin H, Raskin P ; Diabetes mellitus, vol 5, American Diabetes Association, Premtice-Hall International, Maryland, 291-5.
- Cruickshank, R., Duguid, J. P., Masion B. P. and Swain, R. H. (1979), Medical Microbiology. 12th Edition: Churchill Livingstone, Edinburgh, London, New York.
- Gadepalli, R., Dhawan, B., Sreenivas, V., Kapil, A., Ammini, A.C. and Chaudhry, R. (2006). A Clinico-microbiological Study of Diabetic Foot Ulcers in an Indian Tertiary Care Hospital. Diabetes Care, 29: 1727-32.
- Harmita, and Radji, M. (2005). Analysis of microbiology, Edisi Kedua. Jakarta, Pharmacy Department, FMIPA UI.
- Hasan H. (2009) Clinical And Laboratory Aspects Of Diabetic Foot Infection, The Indonesian Journal of Medical Science Volume 2 No.1 January-March.
- Chambers, H. F. (2003). Bactericidal versus Bacteriostatic Antibiotic Therapy : A clinical Mini-Review. Clinical updates infectious disease, Volume VI Issue 4 October 2003
- Jewetz. Melnick. Adelergs. (2005). Medical Microbiology, Ed.5th Jakarta: Salemba Medika.
- Joenoes, Nanizar Zaman. (1994). Ars Prescibendi Rational Prescriptio, Part 3, Surabaya : Airlangga University Press.
- Mycek, M. J. (1997). Lippinscott’s illustrated Reviews : Pharmacology. Raven Publisher, Philadelphia.
- Nanang, F. A. (2008). Pattern of aerob bacteria and sensitivity on gangrene diabetic, University of North Sumatra.
- Rowbathan, J. L., Gibbons, G. W., Kozak, G. P. (1982). The Diabetic Foot. In Clinical Diabetes Mellitus, edit. Kozak GP. Philadelphia: WB Saunders Co; 215 - 228.
- Setiabudy, R. (2007). Pharmacology and Therapy, Ed. 5. Jakarta: Medical Faculty of Indonesia University.
- Suharjono, et al. 1994. The Pharmacy Service of Antibiotic and their Information. Pharmacy Faculty of Airlangga University, Surabaya. P.6.