Main Article Content
Abstract
One of the plants had been used as a medicinal plant in Papua is Pometia Pinnata J.R Forst & G. Forst or the Matoa plant. The aim of this study is to determine the antibacterial activity of the active fraction of Matoa seeds against Escherichia coli bacteria. The positive control and negative control used were ciprofloxacin and dimethyl sulfoxide, respectively. 96% ethanol solvent was used to extract the simplicia of Matoa seeds. Fractionation was carried out using n-hexane, ethyl acetate and water as solvents. The Kirby Bauer diffusion method was used in the antibacterial activity assay at concentrations of 100 mg/mL, 200 mg/mL, 300 mg/mL, 400 mg/mL, and 500 mg/mL. Overall, the inhibition zones obtained from the sequential concentrations above were 8.89 mm, 10.18 mm, 10.34 mm, 10.84 mm, and 11.76 mm respectively. Further, data analysis was performed with One Way ANOVA and the results showed significant differences between concentrations. Data analysis was then continued with the post hoc Tukey HSD test and a concentration of 500 mg/mL was concluded as the optimum concentration.
Keywords
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
References
- Anggraini, W., Nisa, S. C., Ramadhani Da, R., & Ma’arif ZA, B. (2019). Aktivitas Antibakteri Ekstrak Etanol 96 % Buah Blewah (Cucumis melo L. var. cantalupensis) Terhadap Pertumbuhan Bakteri Escherichia coli. Pharmaceutical Journal of Indonesia, 5(1), 61–66.
- Amrie, A., Ivan, S., Anam., & Ramadhanil. (2014). Uji Efektivitas Ekstrak Daun dan Akar Harrisonia perforata Merr. Terhadap pertumbuhan Bakteri Vibro chlorae. Journal of Science and Technology. Vol 3 (3).
- Brooks G. F, Janet S. B, Stephen A.M, Jawetz, Melnick, & Adelbergs. (2005). Mikrobiologi Kedokteran Buku 1 (E. Mudihardi, Kuntaman, Wasito E.B, Mertaniasih N. M, Harsono S, & Alimsardjono L, Eds.). Jakarta: Salemba Medika.
- CLSI (Clinical and Laboratory Standars Institute). (2020). Performance Standars for Antimicrobial Susceptiblity Testing 30 th Ed. Diakses pada 7 Maret 2022.
- Erlyn, P. (2016). Efektivitas Antibakteri Fraksi Aktif Serai (Cymbopogon citratus) terhadap Bakteri Streptococcus mutans. Syifa Medika Jurnal Kedokteran dan Kesehatan. 6 (2). 111-125.
- Evans, S. M., & Cowan, M. M. (2016). Plant products as antimicrobial agents. Cosmetic and Drug Microbiology, 12(4), 205–231.
- Farha, A. K., Yang, Q. Q., Kim, G., Li, H. Bin, Zhu, F., Liu, H. Y., Gan, R. Y., & Corke, H. (2020). Tannins as an alternative to antibiotics. Food Bioscience, 38(September), 100751.
- G. Maria Theopanny D. (2019). Uji Aktivitas Antibakteri Ekstrak N-Heksana, Etil Asetat, Serta Etanol 96% Dari Daun Matoa (Pometia Pinnata J.R & G.Fors) Terhadap Propionibacteri Acnes Dan Escherichia Coli (Unpublished undergraduate thesis), Unversitas Sumatera Utara, Indonesia.
- Jahan, F., Lawrence, R., Kumar, V., & Junaid, M. (2011). Evaluation of antimicrobial activity of plant extracts on antibioticsusceptible. Journal of Chemical and Pharmaceutical Research, 777-789.
- Ngajow, M., Abidjulu, J., & Kamu, V. S. (2013). Pengaruh Antibakteri Ekstrak Kulit Batang Matoa (Pometia pinnata) terhadap Bakteri Staphylococcus aureus secara In vitro. Jurnal Mipa Unsrat Online. 2(2). 128-132.
- Pakpahan, D.T & Sutriningsih. (2020). Uji Aktivitas Antibakteri Fraksi n-heksana, Etil asetat, dan Butanol Daun Petai Cina (Leucaena leucocephala (Lam.) de Wit) terhadap Bakteri Propionibacterium acnes dan Staphylococcus epidermidis Secara In Vitro. Indonesia Natural Research Pharmaceutical Journal. 5(2). 12-19.
- Parashar, U. D, Hummelman, E. G, Bresee, J.S, Miller, M. A, & Glass, R. I. (2003). Global illness and deaths caused by rotavirus disease in children. Emerg Infect Disc. 9(5). 565–572.
- Rahayu, W. P., Nurjanah, S., & Komalasari, E. (2020). Escherichia Coli: Patogenitas, Analiss dan Kajian Risiko. Bogor: IPB Press.
- Sari, R. C., Wijayanti, I., & Agustini, T. W. (2019). The Effectiveness of Melanin from Squid Ink (Loligo sp.) as Antibacterial Agent Against Escherichia coli and Listeria monocytogenes. IOP Conference Series: Earth and Environmental Science, 246(1).
- Sabir, A. (2005). Aktivitas Antibakteri Flavonoid Propolis Trigono sp terhadap bakteri Streptococcus mutans (in vitro). Majalah Kedokteran Gigi. 38(3). 135.
- Setiawan, M., Retroningrum,M.,Yahya F. (2020). Aktivitas Antibakteri Ekstrak Biji Jeruk Siam (Citrus reticulata) pada Bakteri Escherichia coli. Jurnal Bioteknologi & Biosains Indonesia. 2(7). 289-295.
- Tripathi, K. D. (2003). Antimicrobial Drugs: General Consideration. Essentials of Medical Pharmacology. Fifth Edition. Jaypee Brothers Medical Publishers.
- Utami, E. R. (2011). Antibiotika, Resistensi, dan Rasionalitas Terapi. In El-Hayah Jurnal Biologi. 1(4). 124-138.