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Abstract
Background: Dengue Fever (DF) is a viral infection disease transmitted by Aedes aegypti. It is one of Indonesian endemic diseases that reported to occur throughout the year. To break the transmission chain of DF, the use of larvicides is preferred, especially using natural ingredients, such as bay leaves (Syzygium polyanthum). Polyethylene glycol (PEG) as a dispersant may prevent the clumping of material test so that it can be distributed evenly in water which is the medium of growth for larvae. Objectives: To determine the effectiveness of ethanolic extract of bay leaves (EEBL) in addition of PEG diluent on the mortality of Aedes aegypti larvae. Material and Method: Bay leaves as the main material were extracted using 95% of ethanol, and were applied in two variation concentration, 0.75% and 1%, while the diluent added was PEG. The samples used in this study were Aedes aegypti larvae at stages III-IV, with a total of 25 individuals for each treatment group. Evaluation was performed every 6 hours, for 24 hours, then the results were recorded and analyzed using the Kruskall Wallis test and the Mann Whitney test. Result: In both of variation concentration used, at 24 hours of observation it was obtained the mortality of Aedes aegypti larvae was 100%. The p-value obtained for the Kruskall Wallis test was <0.05. From Mann Whitney test, when each of treatment group was compared to the positive control, abate®, the p-value obtained is >0.05, while when they were compared to the negative control, PEG, the p-value obtained is <0.05. Conclusion: 95% ethanolic extract of bay leaves in addition of PEG diluent is effective as Aedes aegypti larvicides. It is also known that EEBL at concentration of 0.75% and 1% in addition of PEG are as effective as abate® as Aedes aegypti larvicides.
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References
- Cahyani, D. N., Asngad, A. (2020). Efektivitas Ekstrak Daun Tembelekan dengan Penambahan Daun Cengkeh Dalam Bentuk Spray Sebagai Insektisida Nabati Terhadap Mortalitas Nyamuk. Seminar Nasional Pendidikan Biologi dan Saintek (SNPBS) ke-V. http://download.garuda.kemdikbud.go.id/article.php?article=2987382&val=26810&title=Efektivitas%20Ekstrak%20Daun%20Tembelekan%20dengan%20Penambahan%20Daun%20Cengkeh%20dalam%20Bentuk%20Spray%20sebagai%20Insektisida%20Nabati%20Terhadap%20Mortalitas%20Nyamuk
- Cania, E. B., Setyaningrum, E. (2013). Uji Efektivitas Larvasida Ekstrak Daun
- Legundi (Vitex trifolia) Terhadap Larva Aedes aegypti. Medical Journal of Lampung University, 2(4). https://juke.kedokteran.unila.ac.id/index.php/majority/article/viewFile/62/61
- de Souza Wuillda, A. C. J., Martins, R. C. C., Costa, F. D. N. (2019).
- Larvicidal activity of secondary plant metabolites in aedes aegypti control:
- An overview of the previous 6 years. Natural Product
- Communications, 14(7), https://journals.sagepub.com/doi/full/10.1177/1934578X19862893
- Diah, Y. M., Zulfikar, Z., Ulfa, I., & Hadifah, Z. (2021). Pemetaan Kasus Demam
- Berdarah Dengue Dan Kepadatan Nyamuk Berdasarkan Sistem Informasi
- Geografis (Sig) Di Wilayah Kerja Puskesmas Lhoknga Kabupaten Aceh
- Besar. SEL Jurnal Penelitian Kesehatan, 8(1), 35-46. https://ejournal2.litbang.kemkes.go.id/index.php/sel/article/view/4399
- Ervina, N. (2014). Uji Aktivitas Ekstrak Etanol Daun Singkong (Manihot utilissima
- Pohl) Sebagai Larvasida Aedes aegypti. Jurnal Mahasiswa PSPD FK
- Universitas Tanjungpura, 1(1). https://jurnal.untan.ac.id/index.php/jfk/article/view/8097
- Fatimah, H., Isnawati, Zubaidah, T. (2022). Daya Bunuh Larutan Tanaman Daun Salam (Syzygium Polyanthum) Terhadap Kematian Larva Aedes sp. Gema
- Lingkungan Kesehatan, 20(1), 71-76. https://gelinkes.poltekkesdepkes-sby.ac.id/index.php/gelinkes/article/view/20
- Harismah, K. (2017). Pemanfaatan Daun Salam (Eugenia polyantha) Sebagai Obat
- Herbal Dan Rempah Penyedap Makanan. Warta, 19 (2), 110-118. https://journals.ums.ac.id/index.php/warta/article/view/2742/0
- Huljani, M., Ahsanunnisa, R. (2019). Pemanfaatan Ekstrak Buah Ketumbar
- (Coriandrum sativum L.) sebagai Larvasida Nabati Nyamuk Aedes aegypti.
- Prosiding Seminar Nasional Sains dan Teknologi Terapan. Vol. 2, No.
- 1. http://semnas.radenfatah.ac.id/index.php/semnasfst/article/view/53
- Hutanu, Daniela; Frishberg, Mark, D.; Guo, Ling; Darie, Costel, C. (2014). Recent Applications of Polyethylene Glycols (PEGs) and PEG Derivatives. Mod Chem Appl 2014, 2:2. https://www.researchgate.net/publication/287444069_Recent_Applications_of_Polyethylene_Glycols_PEGs_and_PEG_Derivatives
- Kementerian Kesehatan Republik Indonesia. (2022). Profil Kesehatan Indonesia
- 2021. https://www.kemkes.go.id/downloads/resources/download/pusdatin/profil-kesehatan-indonesia/Profil-Kesehatan-2021.pdf
- Kumara, C. J., Nurhayani, N., Bestari, R. S., Dewi, L. M., (2021). Efektivitas Flavonoid, Tanin, Saponin dan Alkaloid Terhadap Mortalitas Larva Aedes aegypti. Proceeding of The 13th University Research Colloquium 2021: Kesehatan dan MIPA. http://repository.urecol.org/index.php/proceeding/article/view/1311
- Riyadi, Z., Julizar, J., Rahmatini, R. (2018). Uji Efektivitas Ekstrak Etanol Biji
- Rambutan (Nephelium lappaceum L.) Sebagai Larvasida Alami pada Larva
- Nyamuk Aedes aegypti. Jurnal Kesehatan Andalas, 7(2), 233-239. http://jurnal.fk.unand.ac.id/index.php/jka/article/view/807
- Setyaningsih, N., Swastika, I. (2016). Efektivitas Ekstrak Ethanol Daun Salam
- (Syzygium polyanthum) Sebagai Larvasida Terhadap Larva Nyamuk Aedes
- aegypti. E-Jurnal Medika Udayana. 5 (2). https://ojs.unud.ac.id/index.php/eum/article/view/20924
- Suparyati, S. (2020). Uji Daya Bunuh Abate Berdasarkan Dosis dan Waktu
- Terhadap Kematian Larva Nyamuk Aedes sp dan Culex sp. Pena Jurnal
- Ilmu Pengetahuan dan Teknologi, 34(2), 1-9. https://jurnal.unikal.ac.id/index.php/pena/article/view/1193
- Susiwati, S., Apriani, K. L., Sahidan. (2017). Efektifitas Ekstrak Infusa Daun Salam
- (Syzygium Polyanthum) Sebagai Biolarvasida Nyamuk Aedes sp di Kota
- Bengkulu Tahun 2016. Journal of Nursing and Public Health, 5(1), 60-65. https://jurnal.unived.ac.id/index.php/jnph/article/view/599
- Syamsir, S. (2018). Analisis Spasial Efektivitas Fogging di Wilayah Kerja
- Puskesmas Makroman, Kota Samarinda. Jurnal Nasional Ilmu
- Kesehatan, 1(2). https://journal.unhas.ac.id/index.php/jnik/article/view/5996
- Lumowa, S. V. T., Nova, T. P. (2015). Larvicidal activity of Syzygium
- polyanthum W. Leaf Extract Against Aedes aegypti Larvae. Progress in
- Health Sciences, 5(1), 102-106. https://www.umb.edu.pl/photo/pliki/progress-file/phs/phs_2015_1/102-106_lumanowa.pdf
- Wang, W. H., Urbina, A. N., Chang, M. R., Assavalapsakul, W., Lu, P. L., Chen,
- Y. H., Wang, S. F. (2020). Dengue Hemorrhagic Fever – A Systemic
- Literature Review of Current Perspectives on Pathogenesis, Prevention and
- Control. Journal of Microbiology, Immunology and Infection, 53(6), 963-
- 978. https://pubmed.ncbi.nlm.nih.gov/32265181/
- WHO. (2005). Guidelines for Laboratory and Field Testing of Mosquito Larvicides. World Health Organization Communicable Disease Control, Prevention and Eradication - WHO Pesticide Evaluation Scheme. https://www.who.int/publications/i/item/WHO-CDS-WHOPES-GCDPP-2005.13
- WHO (2022). Dengue and severe dengue. [online] Who.int.
- Available at: https://www.who.int/news-room/fact-sheets/detail/dengue-and-severe-dengue