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Submitted
Oct 22, 2023
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Jan 18, 2025
Published
Mar 1, 2025
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Abstract


Background: Acne is a skin infection that may be induced by Propionibacterium acnes. Treatment of acne using natural ingredients native to Kalimantan, such as Balik Angin (Alphitonia incana (Roxb.) Teijsm. & Binn. ex Kurz), can serve as an alternative therapy. Prior research indicates that leaves macerated with methanol and 70% ethanol solvents can inhibit the growth of P. acnes in the medium category, with a Minimum Inhibitory Concentration (MIC) of 3.2%.
Objectives: The purpose of this research is to compare the effects of different Soxhlet extraction solvents on the antibacterial activity of Balik Angin leaves in inhibiting the growth of P. acnes.
Materials and Methods: Balik Angin leaves were extracted using the Soxhlet method with two different solvents: methanol and 70% ethanol. This was followed by phytochemical screening. The antibacterial activity assay was conducted using the well diffusion method, with sample concentrations of 25.6%, 12.8%, 6.4%, 3.2%, 1.6%, 0.8%, 0.4%, and 0.2%.
Results: Both extracts contained the same phytochemical groups, namely phenols, flavonoids, alkaloids, tannins, saponins, and triterpenoids. The antibacterial activity test results showed that both extracts had the same MIC of 0.8%. However, the 70% ethanol extract of Balik Angin leaves produced a larger average inhibition diameter of 9.61 ± 0.35 mm compared to the methanol extract, which had an average of 9.20 ± 0.22 mm. Nonetheless, there was no significant difference (p > 0.05) between the two extracts across the concentration range of 1.6–25.6%.
Conclusions: The conclusion of this research is that differences in Soxhlet extraction solvents used for Balik Angin leaves can affect their activity as an anti-Propionibacterium acnes agent.

 

Keywords

Alphitonia incana (Roxb.) Teijsm. & Binn. ex Kurz Antibacteria Balik Angin Leaves Propionibacterium acnes Soxhlet

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How to Cite
Ramadhan, H., Forestryana, D., Subareng, A. R. M., & Vebruati, V. (2025). The Effect of Different Soxhlet Extraction Solvents Towards Anti-Propionibacterium acnes Activity of Balik Angin Leaves (Alphitonia incana (Roxb.) Teijsm. & Binn. ex Kurz): Pengaruh Perbedaan Pelarut Ekstraksi Soxhlet Terhadap Aktivitas Anti-Propionibacterium acnes dari Daun Balik Angin (Alphitonia incana (Roxb.) Teijsm. & Binn. ex Kurz). Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 11(1), 1-12. https://doi.org/10.22487/j24428744.2025.v11.i1.16634

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