Main Article Content

Abstract

Background: The concept of synergism is one of the approaches taken to combat the problems related to antibiotic resistance. Objectives: The goal of this study was to determine the synergistic effect of antibacterial activity of a combination of essential oil of Zanthoxylum acanthopodium DC fruit and erythromycin against Streptococcus mutans and Streptococcus pyogenes. Methods: The broth microdilution assay was used to determine the Minimum Inhibitory Concentration (MIC) of Z. acanthpodium DC fruit essential oil and erythromycin. The synergistic effects were assessed using the checkerboard method. Results: MIC value of Z. acanthopodium DC fruit essential oil against both bacteria was 2500 mg/mL. The combination of Z. acanthopodium DC fruit essential oil and erythromycin had a synergistic effect against S. mutans and S. pyogenes with fractional inhibitory concentration index (FICI) values of 0.375 and 0.0872, respectively. Conclusions: These results suggest that a combination of herbal plants and conventional antibiotics could be used as an alternative therapy for bacterial infections.

Keywords

Antibacterial Erythromycin Streptococcus mutans Streptococcus pyogenes Synergistic Zanthoxylum acanthopodium DC

Article Details

How to Cite
Rahmi, E. (2024). Synergistic Effect Antibacterial Activity of The Combination of Andaliman (Zanthoxylum acanthopodium DC) Fruit Essential Oil and Erythromycin against Streptococcus mutans and Streptococcus pyogenes . Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 10(2). https://doi.org/10.22487/j24428744.2024.v10.i2.16977

References

  1. Ács, K., Balázs, V. L., Kocsis, B., Bencsik, T., Böszörményi, A., & Horváth, G. (2018). Antibacterial activity evaluation of selected essential oils in liquid and vapor phase on respiratory tract pathogens. BMC Complementary and Alternative Medicine, 18(1), 1–9. https://doi.org/10.1186/S12906-018-2291-9/TABLES/6
  2. Adiguna, P., & Santoso, O. (2017). PENGARUH EKSTRAK DAUN SERAI (CYMBOPOGON CITRATUS) PADA BERBAGAI KONSENTRASI TERHADAP VIABILITAS BAKTERI STREPTOCOCCUS MUTANS. Jurnal Kedokteran Diponegoro, 6(4), 1543–1550.
  3. Altun, M., & Yapici, B. M. (2022). Determination of chemical compositions and antibacterial effects of
  4. 10
  5. 10
  6. Zubair et al., xxxx; Jurnal Farmasi Galenika (Galenica Journal of Pharmacy) (e-Journal); (x)x: x-x
  7. selected essential oils against human pathogenic strains. Anais Da Academia Brasileira de Ciencias, 94(1). https://doi.org/10.1590/0001-3765202220210074
  8. Andries, J. R., Gunawan, P. N., & Supit, A. (2014). UJI EFEK ANTI BAKTERI EKSTRAK BUNGA CENGKEH TERHADAP BAKTERI Streptococcus mutans SECARA IN VITRO. Jurnal E-GiGi (EG), 2(2).
  9. Balali, G. I., Yar, D. D., & Sylverken, A. A. (2023). Antimicrobial activities of Hibiscus sabdariffa and Aspilia africana against clinical isolates of Salmonella typhi. Scientific African, 20. https://doi.org/10.1016/j.sciaf.2023.e01667
  10. Centers for Disease Control and Prevention. (2019). Antibiotic resistance threats in the United States, 2019. Atlanta, Georgia. https://doi.org/10.15620/cdc:82532
  11. Coates, A. R. M., Hu, Y., Holt, J., & Yeh, P. (2020). Antibiotic combination therapy against resistant bacterial infections: synergy, rejuvenation and resistance reduction. Expert Review of Anti-Infective Therapy, 18(1), 5–15. https://doi.org/10.1080/14787210.2020.1705155
  12. Crevelin, E. J., Caixeta, S. C., Dias, H. J., Groppo, M., Cunha, W. R., Martins, C. H. G., & Crotti, A. E. M. (2015). Antimicrobial Activity of the Essential Oil of Plectranthus neochilus against Cariogenic Bacteria. Evidence-Based Complementary and Alternative Medicine, 2015. https://doi.org/10.1155/2015/102317
  13. El Atki, Y., Aouam, I., El Kamari, F., Taroq, A., Nayme, K., Timinouni, M., … Abdellaoui, A. (2019). Antibacterial activity of cinnamon essential oils and their synergistic potential with antibiotics. Journal of Advanced Pharmaceutical Technology and Research, 10(2), 63–67. https://doi.org/10.4103/japtr.JAPTR_366_18
  14. Han, Y., Chen, W., & Sun, Z. (2021). Antimicrobial activity and mechanism of limonene against Staphylococcus aureus. Journal of Food Safety, 41(5). https://doi.org/10.1111/jfs.12918
  15. Haq, S. U., Wang, L., Guo, W., Aqib, A. I., Muneer, A., Saqib, M., … Liang, J. (2022). Enhancing activity of β-lactam and fluoroquinolones antibiotics by artemisinin and its derivatives against MDR Escherichia coli. Frontiers in Veterinary Science, 9. https://doi.org/10.3389/FVETS.2022.1048531/FULL
  16. Hossain, S., Heo, H., De Silva, B. C. J., Wimalasena, S. H. M. P., Pathirana, H. N. K. S., & Heo, G.-J. (2017). Antibacterial activity of essential oil from lavender ( Lavandula angustifolia ) against pet turtle-borne pathogenic bacteria . Laboratory Animal Research, 33(3), 195. https://doi.org/10.5625/lar.2017.33.3.195
  17. Kamal, H. Z. A., Ismail, T. N. N. T., Arief, E. M., & Ponnuraj, K. T. (2020). Antimicrobial activities of citronella (Cymbopogon nardus) essential oil against several oral pathogens and its volatile compounds. Padjadjaran Journal of Dentistry, 32(1), 1. https://doi.org/10.24198/pjd.vol32no1.24966
  18. Kebede, D., Admas, A., & Mekonnen, D. (2021). Prevalence and antibiotics susceptibility profiles of Streptococcus pyogenes among pediatric patients with acute pharyngitis at Felege Hiwot
  19. 11
  20. 11
  21. Zubair et al., xxxx; Jurnal Farmasi Galenika (Galenica Journal of Pharmacy) (e-Journal); (x)x: x-x
  22. Comprehensive Specialized Hospital, Northwest Ethiopia. BMC Microbiology, 21(1). https://doi.org/10.1186/s12866-021-02196-0
  23. Kintamani, E., Batubara, I., Kusmana, C., Tiryana, T., Mirmanto, E., & Asoka, S. F. (2023). Essential Oil Compounds of Andaliman (Zanthoxylum acanthopodium DC.) Fruit Varieties and Their Utilization as Skin Anti-Aging Using Molecular Docking. Life, 13(3), 754. https://doi.org/10.3390/life13030754
  24. Ledingham, K., Hinchliffe, S., Jackson, M., Thomas, F., & Tomson, G. (2019). Antibiotic resistance: using a cultural contexts of health approach to address a global health challenge. Retrieved from www.euro.who.int
  25. Li, Z. H., Cai, M., Liu, Y. S., Sun, P. L., & Luo, S. L. (2019). Antibacterial Activity and Mechanisms of Essential Oil from Citrus medica L. Var. Sarcodactylis. Molecules, 24(8). https://doi.org/10.3390/molecules24081577
  26. Lira, M. H. P. de, Andrade Júnior, F. P. de, Moraes, G. F. Q., Macena, G. da S., Pereira, F. de O., & Lima, I. O. (2020, May 3). Antimicrobial activity of geraniol: an integrative review. Journal of Essential Oil Research, Vol. 32, pp. 187–197. Taylor and Francis Inc. https://doi.org/10.1080/10412905.2020.1745697
  27. Mabeku, L. B. K., Emmanuel, T., Kouam, J., Zra, T., & Louis, O. E. J. (2013). Synergetic Effects of Plant Extracts and Antibiotics on Vibrio cholerae O1 Strains Isolated From Clinical Specimens. International Journal of Biology, 5(3), 64–72. https://doi.org/10.5539/ijb.v5n3p64
  28. Maftuhah, A., Harnina, S. B., & Mustikaningtyas, D. (2015). PENGARUH INFUSA DAUN BELUNTAS (Pluchea indica) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus epidermidis. Unnes Journal of Life Science, 4(1), 60–65. Retrieved from http://journal.unnes.ac.id/sju/index.php/UnnesJLifeSci
  29. Magi, G., Marini, E., & Facinelli, B. (2015). Antimicrobial activity of essential oils and carvacrol, and synergy of carvacrol and erythromycin, against clinical, erythromycin-resistant Group A Streptococci. Frontiers in Microbiology, 6(MAR). https://doi.org/10.3389/FMICB.2015.00165/ABSTRACT
  30. Meinen, A., Reuss, A., Willrich, N., Feig, M., Noll, I., Eckmanns, T., … Markwart, R. (2021). Antimicrobial Resistance and the Spectrum of Pathogens in Dental and Oral-Maxillofacial Infections in Hospitals and Dental Practices in Germany. Frontiers in Microbiology, 12. https://doi.org/10.3389/fmicb.2021.676108
  31. Mirza, A. U., Khan, M. S., Nami, S. A. A., Kareem, A., Rehman, S., Bhat, S. A., & Nishat, N. (2019). Copper Oxide Nanomaterials Derived from Zanthoxylum armatum DC. and Berberis lycium Royle Plant Species: Characterization, Assessment of Free Radical Scavenging and Antibacterial Activity. Chemistry and Biodiversity, 16(8). https://doi.org/10.1002/cbdv.201900145
  32. Murray, C. J., Ikuta, K. S., Sharara, F., Swetschinski, L., Robles Aguilar, G., Gray, A., … Criollo-Mora, E. (2022). Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. Lancet, 399(10325), 629–655. https://doi.org/10.1016/S0140-6736(21)02724-0
  33. 12
  34. 12
  35. Zubair et al., xxxx; Jurnal Farmasi Galenika (Galenica Journal of Pharmacy) (e-Journal); (x)x: x-x
  36. Muzafri, A. (2019). Uji Aktivitas Antimikroba ekstrak Andaliman (Zanthoxylum acanthopodium DC.) pada Staphylococcus aureus. Jurnal Sungkai, 7(1), 122–126.
  37. Nurlaeni, Y., Iskandar, J., & Junaedi, D. I. (2021). Ethnoecology of Zanthoxylum acanthopodium by local communities around lake Toba, North Sumatra, Indonesia. Biodiversitas, 22(4), 1806–1818. https://doi.org/10.13057/biodiv/d220426
  38. Panichayupakaranant, P., Septama, A. W., & Sinviratpong, A. (2019). Synergistic activity of lawsone methyl ether in combination with some antibiotics and artocarpin against methicillin-resistant Staphylococcus aureus, Candida albicans, and Trychophyton rubrum. Chinese Herbal Medicines, 11(3), 321–325. https://doi.org/10.1016/j.chmed.2019.06.001
  39. Percival, S. L., Williams, D. W., Randle, J., & Cooper, T. (Eds.). (2014). Biofilms in Infection Prevention and Control (1st ed.). Academic Press.
  40. Rai, M. K., & Kon, V. K. (2013). Fighting Multidrug Resistance with Herbal Extracts, Essential Oils and Their Components (M. K. Rai & K. V. Kon, Eds.). United States of America: Academic Press. Retrieved from www.elsevierdirect.com/rights
  41. Sanhueza, L., Melo, R., Montero, R., Maisey, K., Mendoza, L., & Wilkens, M. (2017). Synergistic interactions between phenolic compounds identified in grape pomace extract with antibiotics of different classes against Staphylococcus aureus and Escherichia coli. PLoS ONE, 12(2). https://doi.org/10.1371/journal.pone.0172273
  42. Sitanggang, F. M. C., Duniaji, A. S., & Pratiwi, I. D. P. K. (2019). DAYA HAMBAT EKSTRAK BUAH ANDALIMAN (Zanthoxylum acanthopodium DC)  DALAM ETIL ASETAT TERHADAP PERTUMBUHAN Escherichia coli. Jurnal Ilmu Dan Teknologi Pangan, 8(3), 257–266.
  43. Soudeiha, M. A. H., Dahdouh, E. A., Azar, E., Sarkis, D. K., & Daoud, Z. (2017). In vitro evaluation of the colistin-carbapenem combination in clinical isolates of a. baumannii using the checkerboard, Etest, and time-kill curve Techniques. Frontiers in Cellular and Infection Microbiology, 7(MAY). https://doi.org/10.3389/fcimb.2017.00209
  44. Soulaimani, B., Hidar, N. El, Ben El Fakir, S., Mezrioui, N., Hassani, L., & Abbad, A. (2021). Combined antibacterial activity of essential oils extracted from Lavandula maroccana (Murb.), Thymus pallidus Batt. and Rosmarinus officinalis L. against antibiotic-resistant Gram-negative bacteria. European Journal of Integrative Medicine, 43. https://doi.org/10.1016/j.eujim.2021.101312
  45. Sreepian, A., Popruk, S., Nutalai, D., Phutthanu, C., & Sreepian, P. M. (2022). Antibacterial Activities and Synergistic Interaction of Citrus Essential Oils and Limonene with Gentamicin against Clinically Isolated Methicillin-Resistant Staphylococcus aureus. Scientific World Journal, 2022. https://doi.org/10.1155/2022/8418287
  46. Sun, Y., Chen, S., Zhang, C., Liu, Y., Ma, L., & Zhang, X. (2018). Effects of sub-minimum inhibitory concentrations of lemon essential oil on the acid tolerance and biofilm formation of Streptococcus mutans. Archives of Oral Biology, 87, 235–241. https://doi.org/10.1016/j.archoralbio.2017.12.028
  47. 13
  48. 13
  49. Zubair et al., xxxx; Jurnal Farmasi Galenika (Galenica Journal of Pharmacy) (e-Journal); (x)x: x-x
  50. Susanti, N., Situmorang, E., & Fitri, W. (2020). Effectiveness of the Antibacterial Activity of n-Hexane Andaliman (Zanthoxylum Acanthopodium DC) Extract Against Bacillus subtilis, Salmonella typhi, and Staphylococcus aureus. Journal of Physics: Conference Series, 1462(1). Institute of Physics Publishing. https://doi.org/10.1088/1742-6596/1462/1/012072
  51. Tuyen, T. T., Quan, P. M., Thu Le, V. T., Toan, T. Q., Nghi, D. H., Bach, C. P., … Van, N. T. H. (2021). Chemical Composition, Antimicrobial, and Cytotoxic Activities of Leaf, Fruit, and Branch Essential Oils Obtained From Zanthoxylum nitidum Grown in Vietnam. Natural Product Communications, 16(1), 1–7.
  52. Wijesundara, N. M., Lee, S. F., Cheng, Z., Davidson, R., & Rupasinghe, H. P. V. (2021). Carvacrol exhibits rapid bactericidal activity against Streptococcus pyogenes through cell membrane damage. Scientific Reports, 11(1). https://doi.org/10.1038/s41598-020-79713-0
  53. Yang, J., Song, X., Hu, H., Zhong, W., Cao, R., Xu, Y., & Li, R. (2022). Chemical Composition and Antifungal, Anti-Inflammatory, Antiviral, and Larvicidal Activities of the Essential Oils of Zanthoxylum acanthopodium DC. from China and Myanmar. Molecules, 27(16). https://doi.org/10.3390/molecules27165243
  54. Yanti, & Limas, R. W. (2019). Chemical profiling of zanthoxylum acanthopodium essential oil and its antidiabetic activity. Food Research, 3(5), 422–427. https://doi.org/10.26656/fr.2017.3(5).065
  55. Zhou, X., & Li, Y. (2015). Atlas of oral microbiology : from healthy microflora to disease. China: Elsevier Inc.

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