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Abstract
Bakteri mampu bersimbiosis dengan spons karena memiliki hubungan simbiosis secara mutualisme antara spons dengan bakteri dalam penelitian ini bertujuan memperoleh isolat bakteri dan mengetahui karakteristik bakteri yang bersimbiosis dengan spons, spons diambil di Perairan Teluk Tomini Sulawesi Tengah dengan menggunakan pisau. Sampel kemudian dimasukkan dalam botol steril yang diisi dengan air laut steril dan gliserol 30%. Bakteri pada sampel diisolasi pada media SWC dengan metode spread plate dari hasil isolasi terdapat tiga isolat, isolat terdapat tiga yang isolat yang dilanjutkan untuk ketahap karakterisasi yakni (BS 1, BS 3 dan BS 10).
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