Main Article Content
Abstract
A stability study of previously isolated and selected crude amylase PL-16 has been performed. The purpose of this study was to determine the effect of pH and temperature alterations on the stability of crude amylase enzymes PL-16 produced by thermophilic bacteria found in Pulu hot springs in Central Sulawesi. Stability characterization was carried out by storing and incubating crude amylase for 24 hours and at elevated heat prior to activity assay at varied pH and temperatures. Enzyme activity following characterization was determined by the DNS method and UV-VIS spectrophotometry at a wavelength of 540 nm, using maltose as the standard. The results showed that at storage for 24 hours with pH varied from 5-12, the crude amylase enzyme activity was able to maintain its 85-95% stability in the pH range of 7-9, then decreased in stability at pH of 10-12. While at 2 hours storage in elevated temperature, it maintained 84-85% stability against heat at 70-80 °C, then decreased at higher temperatures. Our results suggested that crude amylase PL-16 is a promising candidate for industrial enzyme application due to its capability to maintain stability in high temperatures and acidic to neutral pH.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).