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Abstract
Analisis karakter genotip kakao (Theobroma cacao L.) pada tingkat molekular (DNA) penting dilakukan sebagai langkah awal dalam pemuliaan tanaman untuk menghasilkan klon kakao unggul. Keanekaragaman kakao di perkebunan rakyat Kabupaten Sigi memiliki variasi fenotip dan genotip yang tinggi. Terdapat lebih dari 12 klon kakao yang dapat ditemukan di perkebunan tersebut. RAPD (Random amplified polymorphic DNA) merupakan salah satu penanda molekuler berbasis PCR yang banyak digunakan dalam mengidentifikasi keragaman tanaman. Informasi mengenai jenis primer untuk analisis RAPD pada tanaman kakao khususnya di wilayah Kabupaten Sigibelum pernah dilaporkan.Hasil penelitian ini menunjukkan ketiga primer yaitu TCH05, AS9870 dan TCM20 dapat digunakan dalam mendeteksi variasi genetik setiap klon, kecuali klon Sulawesi 1 dan Sulawesi 2.
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