Isolasi, Karakterisasi dan Identifikasi Bakteri Asam Laktat Penghasil Α & Β-Galaktosidase Produk Fermentasi Kulit Buah Cempedak [Arthocarpus integer (THUNB.) MERR.] dan Bunga Tigarun [Crataeva nurvala Buch-Ham]

Authors

  • Orryani Lambui Tadulako University
  • Langkah Sembiring Universitas Gadjah Mada
  • S. Rahayu Universitas Gadjah Mada

DOI:

https://doi.org/10.22487/25411969.2015.v4.i2.4481

Keywords:

Bakteri Asam Laktat, α-galaktosidase, β-galaktosidase, mandai, jaruk

Abstract

Bakteri asam laktat (BAL) yang dapat menghasilkan α & β-galaktosidase telah diisolasi dari produk fermentasi kulit buah cempedak (mandai) dan bunga tigarun (jaruk) menggunakan medium MRS dengan metode enrichment dilanjutkan dengan surface spread plate dan dimurnikan dengan metode streak plate. Tahapan isolasi berhasil mengisolasi 73 isolat penghasil asam, kemudian di screening awal berdasarkan karakter kunci BAL. Diperoleh 26 isolat BAL dan dikarakterisasi berdasarkan kemampuannya dalam menghasilkan α dan β-galaktosidase melalui pengukuran aktivitas enzim dalam menghidrolisis subsrat p-nitrophenyl-α-D-galactopyronoside (pNPG) dan o-nitrophenyl-β-D-galactopyronoside (oNPG). Dua isolat terpilih yaitu OLM_10 memiliki nilai aktivitas α-galaktosidase tertinggi yaitu 5,07 U/ml dan aktivitas β-galaktosidase 0,43 U/ml dan OLJ_25 memiliki nilai aktivitas α-galaktosidase 4,11 U/ml dan & aktivitas β-galaktosidase tertinggi 1,06 U/ml. Berdasarkan karakterisasi terhadap 2 isolat terpilih didapatkan hasil bahwa isolat OLM_10 diidentifikasi sebagai anggota spesies Streptococcus thermophilus dan isolat OLJ_25 sebagai anggota spesies Lactobacillus plantarum.

Author Biographies

  • Orryani Lambui, Tadulako University
    Biologi
  • Langkah Sembiring, Universitas Gadjah Mada
    Biologi
  • S. Rahayu, Universitas Gadjah Mada
    Teknologi Pertanian

Downloads

Published

2015-08-17

Issue

Vol. 4 No. 2 (2015): Volume 4 Number 2 (August 2015)

Section

Articles

License

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

    1. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

  1. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).