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Aug 20, 2020
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Sep 17, 2020
Published
Sep 30, 2020
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Abstract

The use of natural products has been widely used as a resource of new bioactive chemical compounds. One of them is the Paku Atai Merah (Angiopteris ferox Copel) tuber which has long been used empirically by the Dayak tribe of East Kalimantan as an anti-cancer. The purpose of this study was to determine the anticancer cytotoxic activity of the extract and fractions of Paku Atai Merah tuber against T47D breast cancer cells in vitro. Extract of Paku Atai Merah tubers was obtained by maceration method using ethanol solvent until obtained the ethanolic extract then fractionated using various solvents to obtain n-hexane, ethyl acetate, and aqueous-ethanol fractions. The cytotoxic effect was carried out based on the MTT assay. Phytochemical screening tests showed positive results for the presence of flavonoid, phenolic, tannin, saponin and steroid compounds. The results of the cytotoxic activity study showed that the ethyl acetate fraction had moderate cytotoxic activity in T47D cancer cells with an IC50 value  of 84.8 µg/ml. Ethanol extract (513.06 µg/ml) and n-Hexane frsction (881.97 µg/ ml) were also included in the weak category. This study indicates that ethyl acetate fraction can be developed as a supportive therapy for breast cancer treatment.


 

Keywords

Cytotoxic Breast Cancer T47D Cell MTT Assay Paku Atai Merah

Article Details

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Author Biographies

Andi Nur Aisyah, Sekolah Tinggi Ilmu Farmasi Makassar

Bagian Farmasetika, Sekolah Tinggi Ilmu Farmasi Makassar.

Syamsu Nur, Sekolah Tinggi Ilmu Farmasi Makassar

Bagian Kimia Farmasi, Sekolah Tinggi Ilmu Farmasi Makassar.

Endang Lukitaningsih, Universitas Gadjah Mada

Departemen Kimia Farmasi, Fakultas Farmasi Universitas Gadjah Mada, Yogyakarta.

Rumiyati Rumiyati, Universitas Gadjah Mada

Departemen Kimia Farmasi, Fakultas Farmasi Universitas Gadjah Mada, Yogyakarta.

Asril Burhan, Sekolah Tinggi Ilmu Farmasi Makassar

Bagian Biologi Farmasi, Sekolah Tinggi Ilmu Farmasi Makassar.

Syafia Mustika Adjara, Sekolah Tinggi Ilmu Farmasi Makassar

Bagian Biologi Farmasi, Sekolah Tinggi Ilmu Farmasi Makassar.

Kurnia Rahim, Sekolah Tinggi Ilmu Farmasi Makassar

Bagian Kimia Farmasi, Sekolah Tinggi Ilmu Farmasi Makassar.

How to Cite
Aisyah, A. N., Nur, S., Lukitaningsih, E., Rumiyati, R., Burhan, A., Adjara, S. M., & Rahim, K. (2020). Efek Sitotoksik Ekstrak dan Fraksi Umbi Paku Atai Merah (Angiopteris ferox Copel) Terhadap Sel Kanker Payudara T47D: Cytotoxic Effects of Paku Atai Merah (Angiopteris ferox Copel) Tuber Extract and Fractions against T47D Breast Cancer Cells. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 6(2). https://doi.org/10.22487/j24428744.2020.v6.i2.15255

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