Formulation and Antioxidant Activity Spray Gel Black Grape Seed Ethanol Extract

Abstract

This study aims to conduct the antioxidant activity test of black grapefruit seed ethanolic extracts and spray gel formulation. Black grapefruit (vitis vinifera L.), The main flavonoid compounds (procyanidolic oligomers) that have antioxidant activity in black grape seeds, are reported to trigger vascular endothelial growth factors along with fibroblast agents. To produce more collagen fibers in accelerating healing. This research started with the maceration using 96% ethanol solvent and an evaporation process. The extract's antioxidant activity was determined using the 1,1-diphenyl-2-picrylhydrazil (DPPH) method. Furthermore, the extracts were formulated into spray gel with variations of carbopol 904, ethanol 96%, glycerine, TEA, methylparaben, fragrance, aquadest, and extract concentrations. Spray gel was tested for antioxidant activity and physical properties, including organoleptic, homogeneity, pH, viscosity, spreadability, adhesion, and spraying pattern. The results showed: The results showed that spray gel black grapefruit seed ethanolic extracts with the best antioxidant activity evaluation results were given by formula 3 with an IC50 value of 104.659ppm. Furthermore, the physical evaluation results showed that the ethanol extract of black grapefruit seed could be formulated into spray gel preparations, where the three spray gel formulas with varying concentrations of black grapefruit seed ethanol extract, 1%, 2%, and 5%, produce slightly yellow-brown to brownish preparations, characteristic of viscous grapefruit liquid, homogeneous, meets the requirements of pH, viscosity, spraying pattern and viscosity dispersion.