Produksi Konsentrat Protein dari Ampas Kelapa (Cocos nucifera L.) Menggunakan NaOH dan (NH4)2SO4 : Production of Protein Concentrate from Coconut Pulp (Cocos nucifera L.) using NaOH and (NH4)2SO4

Hasdinda Hasdinda; Pasjan Satrimafitrah; Syaiful Bahri; Abd. Rahman Razak; Dwi Juli Puspitasari; Indriani; Aini Auliana Amar

doi:10.22487/kovalen.2023.v9.i1.16290

Authors

Hasdinda Hasdinda a:1:{s:5:"en_US";s:40:"Jurusan Kimia FMIPA Universitas Tadulako";}
Pasjan Satrimafitrah Jurusan Kimia FMIPA Universitas Tadulako
Syaiful Bahri Jurusan Kimia FMIPA Universitas Tadulako
Abd. Rahman Razak Jurusan Kimia FMIPA Universitas Tadulako
Dwi Juli Puspitasari Jurusan Kimia FMIPA Universitas Tadulako
Indriani Jurusan Kimia FMIPA Universitas Tadulako
Aini Auliana Amar Jurusan Kimia FMIPA Universitas Tadulako

DOI:

https://doi.org/10.22487/kovalen.2023.v9.i1.16290

Abstract

Coconut pulp wasted from processed coconut oil has a protein content of up to 18.20%. The potential coconut pulp protein can be processed in protein concentrate which has many benefits. This research was conducted to determine the effect of NaOH and (NH₄)₂SO₄ concentrations in isolating protein and producing protein concentrates that have the highest yield and protein content. Isolation of coconut pulp protein was carried out using NaOH at several concentrations, namely 0, 0.2, 0.4, 0.6, 0.8, and 1 M, while the protein concentrate was prepared using the salting out method with a saturation level of the ammonium sulfate salt of 55%, 60%, and 65%. Analysis of protein content was carried out using the Kjeldahl method. The use of NaOH 0.2 M and (NH₄)₂SO₄ with a saturation level of 60% was the chosen treatment with crude protein content and yield of 75.63% and 33.83%, respectively.